LOCUS       40924_25556        5890 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pInSRT-GFPhFDC.SP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Shinomura T, Nakamura S, Ito K, Shirasawa S, Hook M, Kimura JH.
  TITLE     Adsorption of Follicular Dendritic Cell-secreted Protein (FDC-SP) 
            onto Mineral Deposits: APPLICATION OF A NEW STABLE GENE EXPRESSION 
            SYSTEM
  JOURNAL   J. Biol. Chem. 283 (48), 33658-33664 (2008)
  PUBMED    18806264
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2008) Contact:Tamayuki Shinomura Tokyo Medical and
            Dental University, Graduate School, Tissue Regeneration; 1-5-45 
            Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
REFERENCE   3  (bases 1 to 5890)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5890)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "2008"; volume: "283"; issue: "48"; pages: 
            "33658-33664"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JAN-2008) Contact:Tamayuki Shinomura Tokyo Medical and Dental 
            University, Graduate School, Tissue Regeneration; 1-5-45 Yushima, 
            Bunkyo-ku, Tokyo 113-8549, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..32
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    50..97
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             293..1009
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYR"
     misc_feature    1258..1844
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1845..2441
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    2531..2755
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(2789..2807)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2814..2830)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2971..3426)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3760..3864
                     /label=AmpR promoter
     CDS             3865..4722
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4896..5484
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5772..5793
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5808..5838
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5846..5862
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5870..5886
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attacgccaa gctatttagg tgacactata gaatactcaa gcttcgaccg aagttcctat
       61 tccgaagttc ctattctcta gaaagtatag gaacttcgga tccccgggga gtcgacggta
      121 tcgataagct tgcgcagcgc tgcagtgaat tttcccccca aactgcaata agccgccttc
      181 caaggccaag atgttcataa atataaagag catcttatgg atgtgttcaa ccttaatagt
      241 aacccatgcg ctacataaag tcaaagtggg aaaaagccca ccggtcgcca ccatggtgag
      301 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      361 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      421 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      481 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      541 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      601 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      661 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      721 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      781 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      841 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      901 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      961 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacagat ccccagtctc
     1021 tcaagaccag gaacgagaaa aaagaagtat cagtgacagc gatgaattag cttcagggtt
     1081 ttttgtgttc ccttacccat atccatttcg cccacttcca ccaattccat ttccaagatt
     1141 tccatggttt agacgtaatt ttcctattcc aatacctgaa tctgccccta caactcccct
     1201 tcctagcgaa aagtaaacaa gaaggatctc gcgatatctc gagaattccg cccccccccc
     1261 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     1321 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     1381 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     1441 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     1501 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     1561 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     1621 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     1681 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     1741 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     1801 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatgacc gagtacaagc
     1861 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     1921 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     1981 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     2041 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     2101 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     2161 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     2221 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     2281 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     2341 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     2401 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     2461 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gatgatctag agctcgctga
     2521 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     2581 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     2641 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     2701 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     2761 gaggcggaaa gaacctgcag caattcgccc tatagtgagt cgtattacaa ttcactggcc
     2821 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2881 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2941 caacagttgc gcagcctgaa tggcgaatgg aaattgtaaa cgttaatatt ttgttaaaat
     3001 tcgcgttaaa tatttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3061 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3121 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3181 gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg aggtgccgta
     3241 aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3301 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3361 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3421 gcgcgtcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3481 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     3541 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3601 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3661 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     3721 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3781 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3841 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3901 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3961 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4021 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4081 ctgctatgtc atacactatt atcccgtatt gacgccgggc aagagcaact cggtcgccgg
     4141 gcgcggtatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4201 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4261 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4321 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4381 aacgacgagc gtgacaccac gatgcctgta gcaatgccaa caacgttgcg caaactatta
     4441 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4501 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4561 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4621 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4681 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4741 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4801 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4861 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4921 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4981 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5041 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5101 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5161 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5221 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5281 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5341 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5401 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5461 tcaggggggc ggagcctatc gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5521 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5581 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5641 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5701 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5761 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     5821 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     5881 tatgaccatg
//
